Journal: The Journal of Immunology Author Choice

Article Title: Upregulation of P2X7 Exacerbates Myocardial Ischemia–Reperfusion Injury through Enhancing Inflammation and Apoptosis in Diabetic Mice

doi: 10.4049/jimmunol.2200838

Figure Lengend Snippet: Inhibitor and agonist of P2X7 attenuated or aggravated MI/R injury. (A) Representative Western blot shows the effect of BzATP and BBG on P2X7. (B) Quantitative analysis of P2X7 (n = 6). (C) Plasma CK-MB levels (n = 4–8). (D) Infarct size was determined by Evans blue/TTC staining. (E) Postischemia risk area expressed as a percentage of risk area to the total area (n = 3–6). (F) Postischemia infarct size expressed as a percentage of infarct size to the area at risk (n = 3–6). (G) Representative M-mode echocardiographic images. (H) EF (n = 3–8). (I) FS (n = 3–8). (J) Three-day survival rate (n = 9–17). *p < 0.05, ***p < 0.001. DM I/R, diabetic mice with MI/R operation; DM I/R + BBG, diabetic mice with BBG treatment and MI/R operation; Non-DM I/R+BzATP, nondiabetic mice with BzATP treatment and MI/R operation.

Article Snippet: Administration of drugs BBG (S3217, Selleck), a P2X7 receptor antagonist, was diluted at 3 mg/ml in vehicle (saline) solution and injected i.p.

Techniques: Western Blot, Clinical Proteomics, Staining